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retronectin takara cat  (TaKaRa)


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    Structured Review

    TaKaRa retronectin takara cat
    Retronectin Takara Cat, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cat+b/RetroNectin+Recombinant+Human+Fibronectin+Fragment/pm41557504-214-91-92
    Average 97 stars, based on 1126 article reviews
    retronectin takara cat - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Transduction:

    Article Title: Auto‐inducible expression of chimeric antigen receptor T cells using the NR4A1 promoter
    Article Snippet: .. At 24 h prior to T cell transduction, 24‐well plates were coated with 300 μL of 40 μg ml −1 retronectin (catalog number: T100A/B; TAKARA) for 18 h at 4°C. ..

    Article Title: Glucuronoxylomannan (GXM) receptor chimeric antigen receptors and use thereof
    Article Snippet: Blood samples (buffy coats) obtained at MD Anderson Blood Donor Center were used for PBMC isolation by Ficoll-Paque Plus solution (GE Healthcare Life Sciences; Cat. No. 17144002) according to the manufacturer's instructions. .. Transduction of PBMCs was performed using the Retronectin reagent (Takara Bio Inc.; Cat. No. T100A/B) according to the manufacturer's instructions. ..

    Article Title: Auto-inducible expression of chimeric antigen receptor T cells using the NR4A1 promoter.
    Article Snippet: .. At 24 h prior to T cell transduction, 24-well plates were coated with 300 μL of 40 μg ml 1 retronectin (catalog number: T100A/B; TAKARA) for 18 h at 4°C. ..

    other:

    Article Title: Hypermethylation of the p16 INK4a Promoter as an Early Epigenetic Event in Nodular Hyperplasia Associated with Secondary Hyperparathyroidism
    Article Snippet: The primers used were as follows: First-round PCR primers for unmethylated sequence detection: Sense: 5'-AGGAGGGGTTGGTTGGTTATTAGA-3' Antisense: 5'-CAACCCCAAACCACAACCATAA-3' First-round PCR primers for methylated sequence detection: Sense: 5'-AGGAGGGGTTGGTTGGTTATTAGA-3' Antisense: 5'-GACCCCGAACCGCGACCGTAA-3' Second-round PCR primers for unmethylated sequence detection: Sense: 5'-TTATTAGAGGGTGGGGTGGATTGT-3' Antisense: 5'-CAACCATAACCAACCAATCAACCA-3' Second-round PCR primers for methylated sequence detection: Sense: 5'-TTATTAGAGGGTGGGGCGGATCGC-3' Antisense: 5'-CGACCGTAACCAACCAATCAACCG-3' PCR amplification was carried out under the following conditions: For unmethylated-specific PCR: First round: 30 cycles at 60°C annealing temperature Second round: 25 cycles at 60°C annealing temperature For methylation-specific PCR: First round: 30 cycles at 65°C annealing temperature Second round: 25 cycles at 65°C annealing temperature Each PCR reaction was performed in a 20 μL volume containing 1× buffer, 1.5 mM MgCl 2 , 200 μM dNTPs, 0.5 μM of each primer, and 1 unit of MightyAmp DNA Polymerase ver.2 (Cat. No. R071A/B, Takara Bio Inc., Shiga, Japan), which incorporates hot-start antibodies to suppress nonspecific amplification up to 98°C.



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